Dr. Lim is an assistant professor in the Department of Chemical and Biomolecular Engineering, and a secondary member of the Department of Cell and Developmental Biology at the Perelman School of Medicine. She obtained B.S. in Chemical and Biomolecular Engineering from University of Pennsylvania in 2010, and a Ph.D. in Chemical and Biological Engineering from Princeton University in 2015. She worked as an NIH F32 postdoctoral fellow at the Lewis-Sigler Institute of Genomics at Princeton University, before coming to Penn as an assistant professor in 2018.
The Lim lab is interested in the regulation of chemical kinetics in biological systems, especially in understanding how inherently stochastic gene expression dynamics are “tamed” to produce reliable cellular outcomes. The lab focuses on characterizing the normal range of gene expression kinetics that ensures normal development, using a combination of genome editing, quantitative live imaging, and mathematical modeling. We examine how robustness in development is disrupted upon modulation of enhancer-promoter interactions or 3D chromosomal conformations. In complement to largely used genome-wide “seq” data, we believe that direct “images” of transcription during development can help identify design principles of gene regulation. Lim lab routinely uses CRISPR-mediated genome editing to perturb regulatory sequences and 3D genome architecture, and uses RNA live imaging techniques and quantitative analysis to provide a novel perspective on enhancer-mediated transcriptional dynamics. We envision fully characterizing high-spatiotemporal dynamics of transcriptional regulation and examining the effects on developmental and disease phenotypes.
Cellular and Biomolecular Engineering
Ph.D.: Princeton University (2015)
B.S.E.: University of Pennsylvania (2010)
2022 Penn CBE Distinguished Faculty Award
2022 Dean’s Distinguished Visiting Professorship – UPenn
2021 NSF CAREER Award
2020 NIH MIRA R35 Award
2020 KIChE President Young Investigator Award